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以纯化的重组碱性成纤维细胞生长因子为抗原，免疫小鼠取其脾细胞与ＳＰ２／０细胞的融合，获得了多株稳定分泌抗碱性成纤维细胞生长因子单克隆抗体杂交瘤细胞株；并以Ｗｅｓｔｅｒｎｂｌｏｔ和单克隆抗体抑制碱性成纤维细胞生长因子促进ＢＡＬＢ／Ｃ３Ｔ３细胞生长作用以及ＡＢＣ免疫组化分析了抗体的特异性。
Using purified recombinant basic fibroblast growth factor as antigen, the spleen cells of the mice were fused with SP2 / 0 cells to obtain a plurality of hybridoma cell lines stably secreting the monoclonal antibody against basic fibroblast growth factor ; And Westernblot and monoclonal antibodies inhibit basic fibroblast growth factor in BALB / C3T3 cell growth and ABC immunohistochemistry antibody specificity.