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目的应用ROC评价4种低效价抗体的检测方法,通过正交设计优化低效价抗体检测条件。方法分别用盐水法、聚凝胺法、木瓜酶法,微柱凝胶法对216例标本抗体筛查,绘制ROC曲线比较4种检测方法;设计正交试验,优化低效价抗体的检测条件,利用极差分析法寻找最佳检测条件组合。结果盐水法、聚凝胺法、木瓜酶法、微柱凝胶法ROC曲线下面积分别为0.56、0.93、0.90、0.96;正交试验显示,处理筛选细胞的试剂极差最大,为8.0,微柱凝胶法检测低效价抗体评分最高水平分别为反应时间30 min,筛查细胞浓度2%,木瓜酶处理筛选细胞和筛选细胞所含抗原为纯合子。结论微柱凝胶法诊断效率最高,优于其它3种检测方法;该法检测低效价抗体最佳检测条件组合为:反应时间30 min,筛查细胞浓度2%,木瓜酶处理筛选细胞和筛选细胞所含抗原为纯合子。
Objective To evaluate the detection methods of four kinds of low titer antibodies by ROC and to optimize the detection conditions of low titer antibodies by orthogonal design. Methods 216 samples were screened by saline method, polybrene method, papain method and microcolumn gel method respectively, and ROC curves were drawn to compare the four detection methods. The orthogonal test was designed to optimize the detection conditions of low titer antibodies , The use of range analysis to find the best detection conditions combination. Results The area under ROC curve of saline method, polybrene method, papain method and microcolumn gel method were 0.56, 0.93, 0.90 and 0.96, respectively. The orthogonal test showed that the reagents for screening cells had the highest difference of 8.0, The highest titer of antibody with low titer detected by column gel method was reaction time 30 min, screening cell concentration 2%, papaya enzyme treatment screening cells and screening cells containing antigen homozygote. Conclusion The microcolumn gel method has the highest diagnostic efficiency, which is superior to the other three detection methods. The optimum conditions for the detection of the low titer antibody are as follows: reaction time 30 min, screening cell concentration 2%, papain treatment screening cells Screening cells contained antigen is homozygous.