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目的:探讨过度训练大鼠心肌损伤的机制及丹参注射液的干预效应。方法:56只健康雄性SD大鼠随机分为四组:C组、NS组、O组、OSM组。C组常规饲养;NS组进行中等强度游泳训练;O组进行负重过度训练;OSM组训练方案同O组,且从第4周开始每日灌服丹参注射液。各组分别于运动后即刻和24小时检测血清CK-MB活性、心肌肌钙蛋白Ⅰ(cTnⅠ)及肿瘤坏死因子(TNF-α)含量,电镜下观察心肌组织形态学变化。结果:(1)O组、OSM组即刻和24小时血清cTnⅠ、CK-MB及TNF-α均高于C组和NS组(P<0.05),OSM组低于O组(P<0.05)。(2)O组心肌病理组织学和超微结构改变明显,OSM组变化程度轻于O组。结果提示:丹参注射液能有效减轻过度训练大鼠的心肌损伤,其机制可能与减少机体炎症介质的产生与释放有关。
Objective: To investigate the mechanism of myocardial injury in over-training rats and the intervention effect of Salvia miltiorrhiza injection. Methods: Fifty-six healthy male SD rats were randomly divided into four groups: C group, NS group, O group and OSM group. Group C was fed routinely; Group NS was given moderate intensity swimming training; Group O was overloaded with exercise; Group OSM was trained with Group O, and Salvia miltiorrhiza was administered daily from Week 4. Serum CK-MB activity, cardiac troponin I (cTn I) and tumor necrosis factor (TNF-α) levels were measured immediately after exercise and 24 hours after exercise. The morphological changes of myocardium were observed under electron microscope. Results: (1) Serum levels of cTnⅠ, CK-MB and TNF-α in group O and group OSM were significantly higher than those in group C and NS (P <0.05), while group OSM was lower than group O (P <0.05). (2) The histopathological and ultrastructural changes of myocardium in group O were obviously changed. The degree of change in group OSM was lighter than that in group O. The results suggest that Salvia miltiorrhiza injection can effectively reduce myocardial injury in over-trained rats, the mechanism may be related to reducing the production and release of inflammatory mediators in the body.