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目的调查我院医务人员手部葡萄球菌携带情况，查明其增多原因。方法将医务人员手部携带的部分金黄色葡萄球菌、凝固酶阴性葡萄球菌做了“８４”消毒剂定性杀菌试验、ＭＩＣ检测、β－内酰胺酶、耐甲氧西林检测，抗菌药物耐药谱、质粒酶切谱分型。结果发现我院医务人员手部金黄葡萄球菌、葡萄球菌携带率已分别从１９９２年的８７０％（１０／１１５）、３２１７％（３７／１１５）上升至１９９７年的３４０６％（７８／２２９）、６５９４％（１５１／２２９），且７７９７％的菌株为ＭＲＳＡ（或ＭＲＣＮＳ），各科室之间无同源菌株。全部试验菌株在“８４”消毒剂有效氯的含量为１００ｐｐｍ浓度时，１分钟内可将其全部杀灭。结论我院医务人员手部葡萄球菌及金黄色葡萄球菌逐年上升的因素为：①长时间使用“８４”消毒剂，导致了部分菌株对“８４”消毒剂敏感性降低；②医务人员不常洗手；③采用“８４”消毒剂洗手致手部粗糙，难以洗净。
Objective To investigate the situation of carrying staphylococci in the hand of medical staff in our hospital to find out the reasons for the increase. Methods The staphylococcus aureus and coagulase-negative staphylococci carried by the hand of the medical staff were used to conduct the qualitative disinfection test of “84” disinfectant, MIC detection, β-lactamase, methicillin resistance test, antibacterial drug resistance spectrum , Plasmid restriction enzyme digestion. The results showed that the carrying capacity of Staphylococcus aureus and Staphylococcus aureus among medical staff in our hospital increased from 870% (10/115) and 3217% (37/115) in 1992 to 3406% in 1997 respectively. (78/229), 6594% (151/229), and 7797% of the isolates were MRSA (or MRCNS). There was no homologous strain among the departments. All test strains can be completely killed within 1 minute when the effective chlorine content of the “84” disinfectant is 100 ppm. Conclusion The increasing amount of Staphylococcus aureus and Staphylococcus aureus in the hand of medical staff in our hospital are as follows: ① The prolonged use of “84” disinfectant resulted in the decrease of susceptibility of some strains to “84” disinfectant; ② The medical staff did not wash their hands frequently ; ③ using “84” hand sanitizer hand rough, hard to wash.