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AIM:To investigate the inhibitory effect of a Chinese herbmedicine Astragali radix(AR)on growth of different cancercell lines.METHODS:To observe the in vitro effects of AR on tumorcell proliferation by trypan blue exclusion,MTS method andtritium thymidine incorporation assay.Apoptosis wasdetected by DNA ladder method.RESULTS:The inhibition rates of AR on the cell respirationof AGS,KATOIII,HT29,MDA231,MEL7 and MEL14 were68.25 %,62.36 %,22.8 %,27.69 %,2.85%and 5.14 %respectively at the concentration of 100 ug/ml;it inhibitedAGS DNA synthesis by 87.33%at the concentration of 50ug/ml.The inhibitory effect on AGS was time-and dose-dependent.AR did not induce apoptosis in AGS cells.CONCLUSION:AR specifically inhibits gastric cancer cellsgrowth in vitro and the mechanism is mainly cytostatic butnot cytotoxic or inducing apoptosis.
AIM: To investigate the inhibitory effect of a Chinese herbmedicine Astragali radix(AR) on growth of different cancercell lines.METHODS: To observe the in effects effects of AR on tumorcell proliferation by trypan blue exclusion,MTS method andtritium thymidine incorporation assay.Apoptosis was detected By DNA ladder method.RESULTS: The inhibition rates of AR on the cell respiration of AGS, KATOIII, HT29, MDA231, MEL7 and MEL14 were68.25 %,62.36 %,22.8 %,27.69 %,2.85% and 5.14 %respectively at the concentration Of 100 ug/ml;it inhibited AGS DNA synthesis by 87.33% at the concentration of 50ug/ml.The inhibitory effect on AGS was time-and dose-dependent.AR did not induce apoptosis in AGS cells.CONCLUSION:AR specifically inhibits gastric cancer Cellsgrowth in vitro and the mechanism is mainly cytostatic butnot cytotoxic or inducing apoptosis.