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A new method of preparing silk fibroin (SF) solution used in the electerospinning was introduced in this paper. According to the method, SF was dissolved in the LiBr/CH2O2 solution directly at room temperature. The method was compared with the traditional method---SF was dissolved in CaCl2 ternary solution. The structure of SF films and the morphology of SF nanofibers were examined by attenuated total reflectance fourier transform intrared (ATR- FrlR) spectroscopy, Scanning electron microscope (SEM) and optical polarizing microscope. The result of this study shows that the new method is a faster, more convenient and high efficient way to get the SF solution and the characteristics of SF fibet made by the new method is much betty.