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为了应用新型的SRAP分子标记技术分析水稻茄丝核菌基因多态性和探究基因多态性与采集来源及水稻品种之间的关系,以吉林省不同地理位置的3株水稻丝核菌为材料,从100对SRAP引物中筛出较理想的引物16对,并对影响SRAP反应的Mg2+、dNTPs、引物、Taq DNA聚合酶、模板DNA浓度及两次退火温度等条件进行了优化,建立了适宜于该菌的SRAP反应体系,25μL的反应体系包含2.5 mmol/L Mg2+、0.15 mmol/L dNTPs、0.8μmol/L引物、1.5 U Taq DNA聚合酶和80 ng模板DNA。利用24株水稻丝核菌对优化后的SRAP反应体系进行了验证,结果表明该反应体系稳定可靠,能够有效地用于水稻茄丝核菌的遗传多样性分析。
In order to analyze the relationship between the genetic polymorphism of Rhizoctonia solani and Rhizoctonia solani using SRAP molecular marker technology and the relationship between the genetic diversity and the sources and rice varieties, three Rhizoctonia solani isolates from different geographical locations in Jilin Province were used as materials , 16 pairs of ideal primers were screened out from 100 pairs of SRAP primers and the conditions of Mg2 +, dNTPs, primers, Taq DNA polymerase, template DNA concentration and annealing temperature were optimized, For the bacteria SRAP reaction system, 25μL reaction system contains 2.5mmol / L Mg2 +, 0.15mmol / L dNTPs, 0.8μmol / L primer, 1.5U Taq DNA polymerase and 80ng template DNA. The optimized SRAP reaction system was validated by 24 Rhizoctonia solani strains. The results showed that the reaction system was stable and reliable and could be used to analyze the genetic diversity of Rhizoctonia solani.