论文部分内容阅读
目的以汉中市不同县区主栽茶叶品种鲜叶为试验材料,系统分析汉中茶区茶叶中茶氨酸的含量,并对其进行分析评价。方法采用高效液相色谱法(high performance liquid chromatography,HPLC),Diamonsil C18(46 mm×250 mm,5μm);以乙酸铵和乙酸铵、甲醇、乙腈的混合溶液(乙酸铵:甲醇:乙腈=1:2:2,V:V:V)作为流动相,流速:1 mL/min;柱温:25℃;检测波长为338 nm;对不同地区不同品种的茶叶中的茶氨酸的含量进行测定。结果方法的精密度高和重复性好。加标回收率在87.85%~96.95%之间,相对标准偏差为2.25%。汉中茶叶茶氨酸含量总体较高,均值可达3.55 g/100 g。宁强县种植的龙井长叶茶氨酸含量最高,为5.3 g/100 g,远高于群体种茶氨酸最高值4.72 g/100 g。结论就茶氨酸而言,不同品种茶树的差异较大,且新引进品种优于群体种。
Objective To analyze the content of theanine in the tea of Hanzhong tea area by using the fresh leaves of the main varieties of tea in different counties of Hanzhong as experimental materials. Methods High performance liquid chromatography (HPLC) was performed on a Diamonsil C18 (46 mm × 250 mm, 5 μm) column with a mixture of ammonium acetate and ammonium acetate, methanol and acetonitrile (ammonium acetate: methanol: acetonitrile = 1 : 2: 2, V: V: V) as mobile phase, the flow rate was 1 mL / min, the column temperature was 25 ℃, the detection wavelength was 338 nm. The content of theanine in different varieties of tea was determined . The results of the method of high precision and reproducibility. The spiked recoveries ranged from 87.85% to 96.95% with relative standard deviations of 2.25%. Theanine content of Hanzhong tea is generally high with an average value of 3.55 g / 100 g. The content of theanine in Longjing Changye that was planted in Ningqiang County was the highest, 5.3 g / 100 g, much higher than the highest value of 4.72 g / 100 g of theanine. Conclusion In the case of theanine, different varieties of tea tree differences, and the introduction of new varieties better than the group of species.