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目的 :检测感染 HBV饮服人群的 HBV- DNA,研究 HBV- DNA阳性者的家庭聚集性 ,探讨其传染性 ,为修订有关法规、采取有效控制措施提供依据。方法 :用反向间接血凝法检测HBs Ag,用赖氏法检测 AL T,酶联免疫吸附试验检测 HBs Ag、抗 - HBs、抗 - HBc、HBe Ag、抗 - HBe。聚合酶链反应 -微孔杂交法和荧光定量 PCR法检测 HBV- DNA。并对 HBV- DNA阳性者的家庭聚集性进行对照研究。结果 :HBs Ag阳性 10 2 2人 ,阳性率 3.5 8%。其中 HBs Ag≥ 1∶ 5 12或 AL T>4 0者 2 37人 ,占阳性者的 2 3.19%。 AL T<4 0、 HBs Ag<1∶ 5 12 ,且 HBe Ag阳性者 12 7人 ,占16 .18%。在 HBs Ag<1∶ 5 12、AL T<4 0、且 HBe Ag阴性的 6 5 8人中检出 HBV- DNA阳性者 2 34人 ,阳性率 35 .5 6 %。HBV- DNA阳性者的家庭聚集率为 86 .4 9% ,家庭成员 HBV感染率为 6 4 .38% ,相对危险性 6 .5 8。HBV- DNA阳性者的家庭成员 HBV感染呈家庭聚集性。结论 :血清学检验结果同时表现为 HBs Ag<1∶ 5 12、AL T<4 0、HBe Ag阴性、HBV- DNA阳性的在岗饮服人员是 HBV的危险传染源。
OBJECTIVE: To detect the HBV-DNA in HBV-infected population, to study the family aggregation of HBV-DNA positive individuals and to explore the contagiousness of HBV-DNA in order to provide evidences for the revision of relevant laws and regulations and effective control measures. Methods: HBsAg was detected by reverse indirect hemagglutination, ALT by Lai’s method, and HBsAg, anti-HBs, anti-HBc, HBeAg and anti-HBe by enzyme linked immunosorbent assay. Polymerase chain reaction-micropore hybridization and fluorescence quantitative PCR were used to detect HBV-DNA. The HBV-DNA positive family aggregation was controlled. Results: There were 1022 HBsAg positive patients with a positive rate of 3.58%. Among them, 377 were HBsAg≥1: 5 12 or ALT> 40, accounting for 23.19% of the positive ones. ALT <40, HBsAg <1: 512, and HBeAg-positive 12.7, accounting for 16.18%. The positive rate of HBV DNA was 35.66% in 658 HBsAg negative patients with HBsAg <1: 5 12, ALT <40, and HBV negative HBeAg. The incidence of HBV infection was 86.49% in family members with HBV-DNA positive, and 6.44% in family members. The relative risk was 6.58. HBV infection in family members with HBV-DNA positive was family clustering. Conclusion: Serum test results also showed that HBs Ag was <1:12, ALT <40, negative for HBe Ag and HBV-DNA positive in-service drinkers was a dangerous source of HBV infection.