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目的：探讨β内啡肽对谷氨酸单钠诱导的神经毒性的影响．方法：形态学观察、神经元面积图象分析、线粒体膜蛋白结合钙和单细胞内游离钙浓度测定．结果：β内啡肽在０５－５０ｍｇ·ｋｇ－１范围内以剂量依赖方式加剧谷氨酸单钠诱导的下丘脑弓状核神经元损伤，谷氨酸单钠诱导的线粒体膜蛋白结合钙增多可以被β内啡肽２ｇ·Ｌ－１加强，谷氨酸单钠诱导的单细胞内钙浓度升高也被β内啡肽２ｇ·Ｌ－１从３２０±８４提高至５８９±７８ｎｍｏｌ·Ｌ－１．结论：β内啡肽以剂量依赖方式通过加剧胞内钙自稳态失衡来增强谷氨酸单钠诱导的神经毒性．
Objective: To investigate the effect of β-endorphin on monosodium glutamate-induced neurotoxicity. Methods: Morphological observation, neuron area image analysis, mitochondrial membrane protein bound calcium and single intracellular free calcium concentration were measured. Results: βendorphin increased glutamate monosodium-induced hypothalamic arcuate neuron damage in a dose-dependent manner in the range of 05-50 mg · kg-1. Monosodium glutamate-induced mitochondria Membrane protein combined with increased calcium can be β endorphin 2g · L-1 to strengthen, monosodium glutamate-induced single-cell intracellular calcium concentration was β endorphin 2g · L-1 increased from 320 ± 84 To 589 ± 78nmol·L-1. Conclusion: β-endorphin enhances monosodium glutamate-induced neurotoxicity in a dose-dependent manner by exacerbating the imbalance of intracellular calcium homeostasis.