Objective:To observe effect of alprostadil combined with Diammonium glycyrrhizinate on renal interstitial fibrosis in SD rate.Methods:A total of 75 SD rate were randomly divided into A,B,C,D,E groups with 15 in each group.Rats in group A served as the control group received just only but tissue separation without modeling operation,while model of unilateral ureteral obstruction(UUO) was established in B,C,D,E groups.Rats in A,B group were given saline lavage placebo treatment,while rats in C,D,E groups were given dianunonium glycyrrhizinate and alprostadil injection.Five rats were sacrificed 1,2,3 weeks after modeling,serum creatinine level of femoral venous blood was determined.Transforming growth factor- β1(TCF- β1) and concentration of connective tissue growth factor(CTGF) were also detected by using ELISA.Line renal interstitial tissue was taken after HE staining,renal interstitial TGF- β1 and CTGF expression were detected by using immunohistochemical method.Results:Serum creatinine levels of B,C,D,E group at different time points in were significantly higher than that of group A(P<0.05);serum creatinine levels in group B were significantly higher than that of C,D,E group at each time point(P<0.05).Serum creatinine level of Croup E was significantly lower than C,D group after 2,3 weeks(P<0.05).Rate in A group at each time point showed no significant changes in TGF- β1 and CREA concentration in serum and kidney tissues(P>0.05);while serum and kidney tissue TGF- β1,concentration of CREA.expression of rats in B,C,D,E groups showed a gradual increasing trend over time.TCF- β1 and CREF of Group B in serum and kidney tissues at each time point were significantly higher than that of the other groups(P<0.05).TCF- β1 and CREF of Group E in serum and kidney tissues at each time point were significantly lower than that of B,C,D group at all time points in serum and kidney tissues(P<0.05).Conclusions:Alprostadil combined with diammonium glycyrrhizinate can significantly lower the expression of TGF- β1 and CTGF in serum and tissues of SD rat with renal interstitial fibrosis,thus inhibit rat renal interstitial fibrosis process.It has synergy protective effect. Objective: To observe effect of alprostadil combined with Diammonium glycyrrhizinate on renal interstitial fibrosis in SD rate. Methods: A total of 75 SD rate were randomly divided into A, B, C, D, E groups with 15 in each group. A served as the control group received just only but tissue separation without modeling operation, while model of unilateral ureteral obstruction (UUO) was established in B, C, D, E groups. Rats in A, B group were given saline lavage placebo treatment, while rats in C, D, E groups were given dianunonium glycyrrhizinate and alprostadil injection. Five rats were sacrificed 1,2,3 weeks after modeling, serum creatinine level of femoral venous blood was determined. Transforming growth factor-β1 (TCF-β1) and concentration of connective tissue growth factor (CTGF) were also detected by using ELISA. Line renal interstitial tissue was taken after HE staining, renal interstitial TGF- β1 and CTGF expression were detected by using immunohistochemical method. Results: Serum creati nine levels of B, C, D, E group at different time points in were significantly higher than that of group A (P <0.05); serum creatinine levels in group B were significantly higher than that of C, D, E group at each Serum creatinine level of Croup E was significantly lower than C, D group after 2,3 weeks (P <0.05) .Rate in A group at each time point showed no significant changes in TGF-β1 and CREA concentration in serum and kidney tissues (P> 0.05); while serum and kidney tissue TGF- β1, concentration of CREA. Expression of rats in B, C, D, E groups showed a gradual increasing trend trend over time. TCF- β1 and CREF of Group B in serum and kidney tissues at each time point were significantly higher than that of the other groups (P <0.05) .TCF-β1 and CREF of Group E in serum and kidney tissues at each time point were significantly lower than that that of B, C, D group at all time points in serum and kidney tissues (P <0.05) .Conclusions: Alprostadil combined with diammonium glycyrrhizinate can significan tly lower the expression of TGF-β1 and CTGF in serum and tissues of SD rat with renal interstitial fibrosis, thus inhibit rat renal interstitial fibrosis process. It has synergy protective effect.