Ginkgo biloba is a dioecious and unique species within Ginkgo family and Ginkgo genus, but it shows a big variability. From 1950s, resources of female plants in China were investigated and collected, and the seed anatomical structure and morphological characters at different development stages were observed. According to the shape, length, width and thickness of ovule bracket and seed stone, as well as the characteristics of both sides and top of the stone, the female plants of Ginkgo biloba have been divided into five types, long-stone, Buddha-finger, horse-bell, mume-stone and round-stone. In addition, the important cultivars belonging to different types were also determined. The stone weight, index of stone shape, and contents of dry matter, amylose, amylopectin, starch, rough protein, soluble sugar and hydrocyanic acid in seed kernel of each type were measured, and the qualities of seed stone were appraised, which lays a foundation for the improvement and utilization of female plant resources in Ginkgo biloba.