论文部分内容阅读
目的 :建立新分离呼肠病毒感染食蟹猴动物模型。方法 :通过滴鼻和静脉感染两种途径 ,用从SARS患者分离的呼肠病毒BYD1株感染 4只食蟹猴 ,每天观察体温变化 ;每组分别在感染后 7d和 33d各处死 1只 ,进行肺脏组织病理学检查和肺脏等脏器的病毒分离。定期采集血清和粪便 ,分别进行特异性中和抗体的测定和病毒分离。结果 :感染猴于接种病毒后的 3～ 4d均开始出现发热症状 ,持续 4d ,体温最高可达 40 .4℃ ,退热 4d后又陆续出现第二次发热。感染动物均产生抗呼肠病毒的中和抗体 ,静脉组抗体滴度高于滴鼻组。从感染动物的肺、心、肝、脾、肾、脑和淋巴组织以及粪便中均分离出呼肠病毒。肺脏组织病理学检查为间质性肺炎。结论 :食蟹猴实验感染呼肠病毒BYD1株后 ,出现发热、免疫应答、病毒血症和间质性肺炎等一系列临床病理学体征 ,说明食蟹猴可以用作呼肠病毒感染实验动物模型。
Objective: To establish a new isolated reovirus-infected cynomolgus animal model. Methods: Four cynomolgus monkeys were infected with reovirus BYD1 isolated from SARS patients by intranasal and intravenous route of infection. The changes of body temperature were observed daily. Each group was sacrificed on the 7d and 33d after infection, Histopathological examination of lungs and isolation of virus from organs such as lungs. Serum and feces were collected on a regular basis, and specific neutralizing antibodies and virus isolation were performed. Results: Infected monkeys began to develop fever symptoms 3 to 4 days after inoculation of virus, lasting 4 days, with body temperature up to 40.4 ℃. After fever 4 days, they developed second fever. Infected animals produce anti-reovirus neutralizing antibodies, intravenous antibody titer higher than the nasal drops group. Reovirus was isolated from the lungs, heart, liver, spleen, kidney, brain and lymphoid tissues, and stools of infected animals. Pulmonary histopathology is interstitial pneumonia. Conclusion: A series of clinical and pathological signs of fever, immune response, viremia and interstitial pneumonia were found in experimental cynomolgus monkeys infected with BYD1 virus, indicating that cynomolgus monkeys can be used as experimental animal model of reovirus infection .