目的 :寻找复发性鼻息肉的相关基因。方法 :对 3例鼻息肉病患者的鼻息肉组织应用mRNA差异显示逆转录PCR(DD RTPCR)技术筛选出相关基因 ,并对差异片段进行序列分析和鉴定。结果 :在背景一致的条件下比较正常鼻甲黏膜组织与鼻息肉组织两者的扩增产物 ,在 15 0 70 0bp处共发现 11个差异片段 ;其中有 1个片段 (X 1)与第 8号染色体的长臂的一段序列完全同源 ,其上游与下游存在锌指蛋白基因。该片段在鼻息肉组织有高表达。结论 :以PCR为基础 ,用DD RTPCR技术能有效地比较鼻息肉与正常鼻甲黏膜组织或细胞间的差异表达基因 ,可筛选出鼻息肉组织相关基因。与第 8号染色体的长臂的一段序列完全同源的差异片段 ,可能是导致鼻息肉发生的尚未被确认的新基因 Objective: To search for recurrent nasal polyps related genes. Methods: Nasopharyngeal polyp tissues of 3 patients with nasal polyposis were screened out by RT-PCR and reverse transcription polymerase chain reaction (DD RTPCR). The differential fragments were sequenced and identified. Results: Compared with normal nasal mucosa tissue and nasal polyp tissue, 11 differentially expressed fragments were found at 15 0 70 0bp under the same background conditions. Among them, 1 fragment (X 1) and 8 Chromosomal long arm of a sequence is completely homologous, its upstream and downstream zinc finger protein gene. This fragment is highly expressed in nasal polyps. Conclusion: Based on PCR, the differentially expressed genes in nasal polyps and normal nasal mucosa tissues or cells can be effectively compared with DD RTPCR technique to screen the relevant genes of nasal polyps. Differentially identical fragments that are completely homologous to a stretch of the long arm of chromosome 8 may be new, unidentified genes that lead to the development of nasal polyps