论文部分内容阅读
目的探讨转化生长因子(TGFβ1)在硬皮病发病中的作用及其与COLI,COLIII含量的相关性。方法采用免疫组化技术检测7例硬皮病小鼠模型的皮损中TGFβ1蛋白及I,III型胶原蛋白含量,以7例正常小鼠皮肤组织作为对照(不加BLM的PBS液处置);并对I,III型胶原及TGFβ1的含量进行比较和相关分析。结果TGFβ1及I,III型胶原在硬皮病小鼠皮损的含量均高于对照组(P<0.001);相关分析表明I型胶原的含量与TGFβ1的含量呈正相关(P<0.05)。结论在硬皮病小鼠皮损中有较多的TGFβ1,且硬化皮肤中TGFβ1与I型胶原的含量有显著相关性。提示其在小鼠皮肤硬化过程中起一定作用,可能与硬皮病的病理纤维化的形成有关。
Objective To investigate the role of TGFβ1 in the pathogenesis of scleroderma and its relationship with COLI and COLIII. Methods Immunohistochemistry was used to detect the levels of TGFβ1 and collagen type I and III collagen in skin lesions of 7 mice with scleroderma. The skin of 7 normal mice was used as a control (untreated with PBS without BLM). The levels of collagen type I, collagen III and TGFβ1 were compared and analyzed. Results The levels of TGFβ1 and collagen type I and III were significantly higher in scleroderma mice than those in control group (P <0.001). Correlation analysis showed that the content of TGF-β1 was positively correlated with TGFβ1 (P <0.05). Conclusion There is more TGFβ1 in the skin lesions of scleroderma mice, and the content of TGFβ1 in sclerotic skin is significantly correlated with the type I collagen. Suggesting that it plays a role in the process of mouse skin sclerosis and may be related to the formation of pathological fibrosis of scleroderma.