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早在1893年Blum就开始应用甲醛作显微镜标本的固定剂,之后已广泛应用于光镜和电镜,至今已有10O年的历史。无论是单一固定剂或是混合固定剂,其浸透组织的速度均较慢,大块组织内部因固定不透而产生自溶现象是常见的。固定的目的是在短时间内,以化学试剂(固定液)与生物细胞组织中的蛋白质和酶相结合,产生不溶性沉淀,以保持组织细胞的生态结构与酶的活性。微波照射(microwave irradiaion,MWI)发热原理就是使照射场中物体(标本)的极性分子产生高速运动,摩擦生热,从而使物品发热,MwI就是以此为特点应用于生物标本固定。Mayers(1970)最先应用
As early as 1893, Blum began to use formaldehyde as a fixative for microscopic specimens, and has been used in light and electron microscopes for a long time, reaching over 10 years. Whether single fixatives or mixed fixatives, are slow to infiltrate tissue and autolysis is common within large tissue due to impermeability. The purpose of immobilization is to combine chemical reagents (fixatives) with proteins and enzymes in biological cell tissues in a short period of time to produce insoluble precipitates that maintain the biological structure and enzyme activity of the tissue cells. The principle of microwave irradiaion (MWI) heating is that the polar molecules of the object (specimen) in the irradiation field produce high-speed movement and generate heat due to the frictional heat generation, and MwI is the characteristic used for the fixation of the biological specimen. Mayers (1970) first application