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目的了解辽宁省现阶段流行的风疹野病毒基因特征,为风疹的控制和消除提供科学依据。方法用Vero/Slam细胞从2007-2008年采集的风疹暴发和散发患者标本中分离到22株风疹野病毒(Rubella Virus,RV),应用逆转录-聚合酶链反应(RT-PCR)针对RV分离株的E 1基因739个核苷酸片段进行PCR扩增,再对扩增产物进行核苷酸序列测定,与世界卫生组织(WHO)RV基因型参考株进行分子流行病学比较分析。结果核苷酸和氨基酸同源性比对及构建亲缘关系树结果显示,22株RV分离株属1 E基因型,核苷酸和氨基酸同源性分别为97.6%～100.0%和99.1%～100.0%;与1 E基因型参考株序列的核苷酸和氨基酸同源性分别为97.0%～99.1%和99.1%～100.0%结论辽宁省分离的22株RV均为1 E基因型,并且可能是辽宁省现阶段的优势基因型。
Objective To understand the epidemiological characteristics of the wild rubella virus in Liaoning Province and to provide a scientific basis for the control and elimination of rubella. Methods Twenty-two strains of Rubella Virus (RV) were isolated from patients with rubella outbreaks and sporadic samples collected from 2007-2008 in Vero / Slam cells. RV isolation was performed by reverse transcription-polymerase chain reaction (RT-PCR) The 739 nucleotide fragment of E 1 gene was amplified by PCR. The amplified products were further sequenced and compared with WHO genotype reference strains for molecular epidemiology. Results Nucleotide and amino acid homology alignment and construction of phylogenetic tree showed that 22 isolates belonged to genotype 1E with nucleotide and amino acid homology of 97.6% -100.0% and 99.1% -100.0, respectively %. The nucleotide and amino acid homologies of the 1 E genotype reference strains were 97.0% -99.1% and 99.1% -100.0%, respectively. CONCLUSIONS: All 22 RV isolates in Liaoning province were of 1E genotype and may be Liaoning Province at this stage the dominant genotype.