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端粒是真核生物染色体末端的多功能特异性DNA-蛋白结构,覆盖在染色体末端,保护基因组的稳定性。端粒在减数分裂过程中起到了十分重要的作用,协助染色体配对、联会、同源重组和分离。精子中的端粒可能在精子的受精能力和胚胎发育中起到重要作用。近年来,端粒与生殖的相关性研究成为一个新的热点,但精子端粒与男性不育间的相关性并不明确。本文采用实时荧光定量PCR方法检测中国特发性男性不育人群(126例)和正常可育男性人群(138例)的精子相对端粒长度,结果发现,特发性男性不育病例的精子平均相对端粒长度(2.894±0.115)低于正常对照组(4.016±0.603),差异具有统计学意义(P=5.097×10?5);并且精子相对端粒长度与精子密度、精子总数和精子活力都有显著的相关性:精子数量较多和/或精子活力较高,精子相对端粒长度较长。研究结果提示,在中国人群中,精子端粒长度与特发性男性不育具有相关性,精子的端粒长度可能影响精子发生和精子的功能,精子端粒的缩短导致精子数目及活力的降低从而导致男性不育。
Telomeres are multifunctional, specific DNA-protein structures at the ends of eukaryotic chromosomes that coat the ends of the chromosomes and protect the genome’s stability. Telomeres play a very important role in meiosis, facilitating chromosome pairing, association, homologous recombination and isolation. Telomeres in sperm may play an important role in sperm fertility and embryo development. In recent years, telomere and reproductive research has become a new hot spot, but the correlation between sperm telomere and male infertility is not clear. In this paper, the relative telomere length of sperm in Chinese idiopathic male infertility population (126 cases) and normal fertile male population (138 cases) was detected by real-time fluorescence quantitative PCR. The results showed that the average sperm motility of idiopathic male infertility cases The relative telomere length (2.894 ± 0.115) was lower than that of the normal control group (4.016 ± 0.603), the difference was statistically significant (P = 5.097 × 10 -5); and the relative telomere length and sperm density, sperm count and sperm motility There is a significant correlation: more sperm and / or higher sperm motility, sperm relative telomere longer. The results suggest that telomere length in sperm is associated with idiopathic male infertility in Chinese population. The telomere length of sperm may affect the function of spermatogenesis and sperm, and the sperm telomere shortening leads to the decrease of sperm number and vitality Resulting in male infertility.