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目的和方法：血管内皮生长因子（ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＶＥＧＦ）是新近确定的一种特异作用于血管内皮细胞的活性肽。最近发现正常心肌细胞可表达ＶＥＧＦ，高血压肥大心脏心肌ＶＥＧＦ及基因表达增强，但对运动性心肌肥大时的变化尚不清楚。本实验采用免疫组化和分子杂交方法，对游泳运动１０周大鼠稳定期肥大心脏心肌ＶＥＧＦ及其基因表达进行研究。结果：ＷｉｓｔａｒＫｙｏｔｏ（ＷＫＹ）大鼠、自发性高血压大鼠（ｓｐｏｎｔａｎｅｏｕｓｌｙｈｙｐｅｒｔｅｎｓｉｖｅｒａｔｓ，ＳＨＲ）和运动大鼠心肌细胞浆内均有特异性ＶＥＧＦ染色颗粒，但运动大鼠心肌细胞胞浆内染色颗粒增加最明显。Ｎｏｒｔｈｅｒｎ分子杂交结果表明三组大鼠心肌均有ＶＥＧＦｍＲＮＡ表达，其中ＳＨＲ表达最强，运动大鼠比ＷＫＹ大鼠增强，但低于ＳＨＲ。结论：目前对这一结果的生理意义还不清楚，推测可能与心肌肥大时细胞间质血管增生有关。
Purpose and Methods: Vascularendothelial growth factor (VEGF) is a recently identified active peptide that acts specifically on vascular endothelial cells. Recently found that normal cardiomyocytes can express VEGF, hypertensive hypertensive heart myocardial VEGF and gene expression increased, but changes in exercise-induced cardiac hypertrophy is not yet clear. In this study, we used immunohistochemistry and molecular hybridization method to study the expression of VEGF and its gene in hypertrophic myocardium of stable rats during swimming 10 weeks. RESULTS: There were specific VEGF-stained particles in WistarKyoto (WKY) rats, spontaneously hypertensive rats (spontaneous hypertensive rats, SHR) and in the myocardial cells of exercise-induced rats. However, the number of stained granules in the cytoplasm of trained rats increased The most obvious. The result of Northern hybridization showed that the expression of VEGF mRNA in myocardium of all the three groups was the strongest, while the expression of SHR was the strongest in exercise-induced exercise than in WKY, but lower than that in SHR. Conclusion: The current physiological significance of this result is not yet clear, suggesting that myocardial hypertrophy may be related to interstitial vascular proliferation.