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目的 观察上皮性钙粘素 (E cadherin)和组织蛋白酶D (CathepsinD)在乳腺导管癌中的表达并分析其与肿瘤侵袭及腋下淋巴结转移的关系。方法 应用免疫组织化学方法检测E cadherin和CathepsinD在乳腺导管癌组织中的表达。结果 乳腺导管原位癌 (carcinomainsitu ,CIS)组织中E cadherin的表达与浸润性导管癌 (infiltratingductalcarcinoma ,IDC)相比无明显差异 (P >0 0 5 )。乳腺导管癌腋下淋巴结阴性组 (nodenegativeductalcarcinoma,NNDC)中E cadherin的表达与腋下淋巴结阳性组 (nodepositiveductalcarcinoma ,NPDC)相比差异不明显 (P >0 0 5 )。乳腺导管癌间质中CathepsinD的表达CIS与IDC相比差异显著 (P <0 0 1) ,NNDC与NPDC相比差异显著 (P <0 0 5 ) ;而在癌细胞中CathepsinD的表达在上述两组中差异均不明显 (P >0 0 5 )。结论 E cadherin在乳腺导管癌的表达与肿瘤的侵袭及淋巴结转移无相关关系。CathepsinD在乳腺导管癌间质的表达与肿瘤的侵袭及淋巴结转移密切相关 ,可作为临床判定肿瘤恶性程度的一个参考指标。
Objective To observe the expression of E cadherin and Cathepsin D in ductal carcinoma of the breast and to analyze their relationship with tumor invasion and axillary lymph node metastasis. Methods Immunohistochemistry was used to detect the expression of E cadherin and Cathepsin D in breast ductal carcinoma tissues. Results There was no significant difference in the expression of E cadherin between infiltrating ductal carcinoma (IDC) and ductal carcinoma (CIS) in the ductal carcinoma of the breast (P> 0.05). The expression of E cadherin in nodenegative ductal carcinoma (NNDC) of breast ductal carcinoma was not significantly different from that in nodepositive ductal carcinoma (NPDC) (P> 0.05). The expression of Cathepsin D in interstitial cells of breast ductal carcinoma was significantly different from that of IDC (P <0.01), but there was significant difference between NNDC and NPDC (P <0 05); while the expression of Cathepsin D in cancer cells was significantly higher than that of IDC There was no significant difference between the two groups (P> 0.05). Conclusion The expression of E cadherin in breast ductal carcinoma has no correlation with tumor invasion and lymph node metastasis. The expression of CathepsinD in breast ductal carcinoma interstitial is closely related to tumor invasion and lymph node metastasis, which can be used as a reference index to determine the degree of tumor malignancy clinically.