建立了可用于微量腹泻性贝毒(diarrhetic shellfish poisoning,DSP)毒素分析的高效液相色谱-质谱联用(high performance liquid chromatography-mass spectrometry,LC-MS)分析方法,并于2009年4月从青岛近海团岛湾采集浮游植物样品,应用所建立的LC-MS方法对手工挑取的渐尖鳍藻(Dinophysis acuminata)细胞产毒状况进行了分析。结果显示,该方法具有良好的灵敏度和精密度,能够对30个鳍藻细胞中的毒素成分进行检测。运用该方法从渐尖鳍藻样品中检测到了大田软海绵酸(okadaic acid,OA)、鳍藻毒素1(dinophysistoxin 1,DTX1)和扇贝毒素2(pectenotoxin 2,PTX2)等毒素成分,细胞中OA,DTX1和PTX2毒素的含量分别为2.54 pg/cell,4.04 pg/cell和1.73 pg/cell,这是首次对我国近海鳍藻产毒状况的报道。 A high performance liquid chromatography-mass spectrometry (LC-MS) method for the analysis of diarrhetic shellfish poisoning (DSP) toxins was established. In April 2009, Phytoplankton samples were collected from the islands of Bay of Islands in Qingdao. LC-MS method was used to analyze the toxin production of hand-picked cells of Dinophysis acuminata. The results show that the method has good sensitivity and precision, and can detect toxin components in 30 fin algae cells. The method was used to detect toxin components such as Okadaic acid (OA), dinophysistoxin 1 (DTX1) and pectenotoxin 2 (PTX2) , And the contents of DTX1 and PTX2 toxin were 2.54 pg / cell, 4.04 pg / cell and 1.73 pg / cell, respectively. This is the first report of the toxin production status of the Chinese coastal dipper.