骨肉瘤是最常见的骨原发性恶性肿瘤,好发于儿童及青少年患者。目前其细胞及分子发病机制尚不明确。随着肿瘤干细胞理论的发展,越来越多的研究证实骨肉瘤干细胞与肿瘤的侵袭、转移、复发及耐药之间也存在联系。因此,本文就骨肉瘤干细胞的存在证据与来源、分选与鉴定方法、小生态环境(niche)以及骨肉瘤干细胞与耐药间的关系的研究进展做一综述。 Osteosarcoma is the most common bone malignancy, occurs in children and adolescents. At present, the pathogenesis of its cells and molecules is not yet clear. With the development of cancer stem cell theory, more and more studies confirm that there is a relationship between osteosarcoma stem cells and tumor invasion, metastasis, recurrence and drug resistance. Therefore, this review summarizes the advances in the evidence of the existence of osteosarcoma stem cells and their relationship with sources, methods of sorting and identification, niche and osteosarcoma stem cells and drug resistance.