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本文介绍了外周血淋巴细胞(PBL)形成自身花环(A-R)的检测方法。“自身花环”是由人淋巴细胞(LC)在体外与自身红细胞(ARBC)结合而成。 作者按Caraux的方法改良,取10~7/毫升的LC0.2毫升与0.05毫升自身血清置4℃预先孵育30分钟,再加入3×10~8/毫升的ARBC0.05毫升,低速离心后放于4℃冰箱孵育过夜,次日轻轻混悬沉淀斑块,计数200只LC中A-C(一只LC结合至少三只ARBC)形成数。以此法测定了122名正常
This article describes the detection of peripheral blood lymphocytes (PBLs) forming their own rosette (A-R). The “native garland” is a combination of human lymphocytes (LCs) and their own red blood cells (ARBCs) in vitro. The authors modified by the method of Caraux, take 10 ~ 7 / ml of LC0.2 ml and 0.05 ml autologous serum pre-incubated at 4 ° C for 30 minutes before adding 3 × 10 ~ 8 / ml of ARBC0.05 ml, low speed centrifugation Incubate overnight at 4 ° C in a refrigerator, gently pellet the pelleted plaques the next day, and count the number of ACs in 200 LCs (one LC in combination with at least three ARBCs). This method measured 122 normal