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目的:通过天麻素鼻腔温敏原位凝胶对蟾蜍口腔黏膜纤毛运动影响的考察,并通过研究其对大鼠鼻腔黏膜的刺激性,评价其鼻腔应用的安全性。方法:将实验动物随机分为生理盐水组、天麻素原位凝胶组、空白凝胶基质组和脱氧胆酸钠组,采用在体蟾蜍上腭法评价各组的纤毛毒性;将实验动物随机分为生理盐水组、天麻素原位凝胶组和空白凝胶基质组,通过大鼠鼻黏膜形态学检查,结合大鼠行为学变化评价各组的鼻黏膜刺激性。结果:与生理盐水组相比,天麻素原位凝胶和空白凝胶基质对蟾蜍口腔纤毛运动的影响无显著差异(P>0.05);生理盐水、天麻素原位凝胶和空白凝胶基质对蟾蜍口腔纤毛运动的影响与脱氧胆酸钠组相比差异有统计学意义(P<0.01)。在整个给药期间及停药后,各组大鼠均未出现打喷嚏现象;与生理盐水组相比,天麻素原位凝胶组和空白凝胶基质组的挠鼻次数均有显著增加(P<0.05),而后两组间无明显差异(P>0.05);停药2 d后,与末次给药时相比,麻素原位凝胶组和空白凝胶基质组的挠鼻次数显著降低(P<0.05),与生理盐水组相比无显著差异(P>0.05);与生理盐水组相比,天麻素原位凝胶组和空白凝胶基质组的炎性细胞浸润明显增多,且伴有充血及纤毛脱落现象;停药后,给药组及基质组的黏膜形态与生理盐水组相比无明显差异。结论:天麻素鼻腔温敏原位凝胶局部应用的安全性较高,值得进一步研发。
OBJECTIVE: To investigate the effect of gastrodin nasal thermosensitive in situ gels on the movement of buccal mucosal ciliary muscle of Toad and to evaluate the nasal mucosa irritation and evaluate the safety of nasal mucosa. Methods: The experimental animals were randomly divided into normal saline group, gastrodin in situ gel group, blank gel matrix group and sodium deoxycholate group. The ciliary toxicity of each group was evaluated by the toad palate method. The experimental animals were randomly The rats were divided into normal saline group, gastrodin in situ gel group and blank gel matrix group. Nasal mucosa irritation was evaluated by morphological examination of nasal mucosa in rats and behavioral changes. Results: Compared with saline group, there was no significant difference in buccal ciliary motility of Bufo bufo gargarizans in situ gel and blank gel matrix (P> 0.05). Compared with normal saline, gastrodin hydrogels and blank gel matrix The effect of buccal ciliary movement in toad was significantly different from that in sodium deoxycholate group (P <0.01). During the whole administration and after stopping, no sneezing occurred in all the rats in each group. Compared with the saline group, the number of flexibilities of gastrodin in situ gel group and blank gel matrix group increased significantly P <0.05), while there was no significant difference between the two groups (P> 0.05). After 2 days’ withdrawal, the times of flexing nose in the group of in situ gel and blank gel matrix were significantly (P <0.05). Compared with the saline group, the inflammatory cell infiltration of gastrodin gel group and blank gel matrix group were significantly increased (P> 0.05) Accompanied by hyperemia and cilia shedding. After stopping the treatment, there was no significant difference in mucosal morphology between the drug-treated group and the matrix-treated group as compared with the saline group. Conclusion: The topical application of gastrodin nasal thermosensitive in situ gel is safe and worthy of further research and development.