目的:建立高效液相色谱法测定注射用曲克芦丁含量的方法。方法:采用LiChrospher 100 RP-18e(5 μm,4.0 mm ×250 mm)色谱柱,以乙腈-四氢呋喃-0.01 mol·L-1磷酸二氢钾溶液(14:6:80)(用磷酸调pH为3.5)为流动相;流速1.0 ml·min-1;检测波长为254 nm。结果:曲克芦丁在47μg·ml-1～330μg·ml-1浓度范围内与峰面积呈良好的线性关系,r= 0.999 99。平均回收率为99.8%,RSD为0.61%,n=9。结论:该方法简便、灵敏、准确,可用于注射用曲克芦丁的质量控制。 Objective: To establish a method for the determination of troxerutin for injection by high performance liquid chromatography. METHODS: LiChrospher 100 RP-18e (5 μm, 4.0 mm × 250 mm) column was used to elute with a mixture of acetonitrile-tetrahydrofuran-0.01 mol·L-1 potassium phosphate monobasic (14: 6: 80) 3.5) as mobile phase; the flow rate was 1.0 ml · min-1; the detection wavelength was 254 nm. Results: Troxerutin showed a good linear relationship with the peak area in the range of 47μg · ml-1 ~ 330μg · ml-1, r = 0.99999. The average recovery was 99.8% with a RSD of 0.61% and n = 9. Conclusion: The method is simple, sensitive and accurate and can be used for the quality control of troxerutin for injection.