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目的:探讨孕酮(P4)处理的树突状细胞(dendritic cell,DC)在诱导同种异基因移植免疫耐受中的作用。方法:实验小鼠分为4组,每组动物供体为C57BL/6雄性小鼠8只,受体为BALB/c雄性小鼠8只。体外培养供体小鼠骨髓来源DC,然后用P4处理。受体小鼠在皮肤移植前经尾静脉分别输注体外培养且经P4处理的供体小鼠DC、供体小鼠成熟DC以及不成熟DC,输注磷酸盐缓冲液为对照组。1周后受体小鼠进行同种异基因皮肤移植,手术后观察皮肤存活情况,应用流式细胞术检测手术前后受体小鼠外周血中CD4+CD25+调节性T细胞(CD4+CD25+T)百分率的变化。结果:在同种异基因皮肤移植中,受体小鼠尾静脉输注经P4处理的DC组分别与成熟DC组、不成熟DC组和对照组比较,移植皮肤存活时间显著延长(P<0.01);与成熟DC组、不成熟DC组和对照组比较,P4处理组外周血中CD4+CD25+T百分率显著升高(P<0.01)。结论:P4处理的DC可以延长同种异基因移植物存活时间。
Objective: To investigate the role of dendritic cell (DC) treated with progesterone (P4) in the induction of immune tolerance to allogeneic transplantation. Methods: The experimental mice were divided into 4 groups: 8 male C57BL / 6 donors and 8 male BALB / c mice. Donor mouse bone marrow-derived DCs were cultured in vitro and then treated with P4. Recipient mice were transfused in vitro with P4-treated donor mouse DCs, donor mouse mature DCs and immature DCs, respectively, via the tail vein prior to skin transplantation. Phosphate buffered saline was used as a control. After 1 week, the recipient mice were allotransplantated with allogeneic skin. The survival of the skin was observed after operation. The levels of CD4 + CD25 + regulatory T cells (CD4 + CD25 + T cells) in peripheral blood of recipient mice before and after operation were detected by flow cytometry ) Percentage change. RESULTS: In allogeneic skin grafts, the survival time of transplanted skin was significantly prolonged (P <0.01 compared with mature DC group, immature DC group and control group) ). Compared with mature DC group, immature DC group and control group, the percentage of CD4 + CD25 + T in peripheral blood of P4-treated group was significantly increased (P <0.01). Conclusions: P4-treated DCs prolong allogeneic graft survival.