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本文建立了一种反相高效液相色谱（ＨＰＬＣ）测定环丙沙星（Ｃｉｐｒｏｆｌｏｘａｃｉｎ，简称ＣＰＦ）血药浓度的方法。固定相为５μｍＵｌｆｒａｓｐｈｅｒｅ－ＯＤＳ（２５ｃｍ×４．６ｍｍ不锈钢柱），流动相为柠檬酸（２０．８２ｍｍｏｌ／Ｌ），醋酸钠（４８．７８ｍｍｏｌ／Ｌ）与０．１％的三乙胺组成的缓冲液：乙腈（８０：２０），流速１ｍｌ／ｍｉｎ，室温ＵＶ检测波长２７８ｎｍ。线性范围０．１２５～１６．０μｇ／ｍｌ，ｒ＝０．９９９７，最低检测限０．０２５μｇ／ｍｌ。５例感染病人的ＣＰＦ药时曲线用非房室模型拟合动力学参数结果：ＡＵＣ＝１１．１±２．８ｍｇ／（ｈ·Ｌ），ＭＲＴ＝４．７±１．１ｈ，Ｔ１／２ＢＥＴＡ＝３．５±０．９ｈ，ＣＬ（Ｔ）＝１９±４Ｌ／ｈ，Ｖｓ＝７６±８Ｌ。
This article established a method for the determination of plasma concentration of Ciprofloxacin (CPF) by reversed-phase high performance liquid chromatography (HPLC). The buffer consisted of 5 μm Ulfrasphere-ODS (25 cm × 4.6 mm stainless steel column) with a mobile phase of citric acid (20.82 mmol / L), sodium acetate (48.78 mmol / L) and 0.1% Liquid: acetonitrile (80:20), flow rate 1ml / min, room temperature UV detection wavelength 278nm. The linear range of 0.125 ~ 16.0μg / ml, r = 0.9997, the minimum detection limit of 0.025μg / ml. The curve of kinetic parameters of CPF in 5 patients was fitted with non-compartmental model to fit kinetic parameters: AUC = 11.1 ± 2.8mg / (h · L), MRT = 4.7 ± 1.1h, T1 / 2BETA = 3.5 ± 0.9 h, CL (T) = 19 ± 4 L / h, Vs = 76 ± 8 L.