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目的:观察末端病大鼠跟腱末端区内皮型一氧化氮合酶(eNOS)的分布,探讨eNOS与血管新生的关系及其在末端病中对机体的保护作用。方法:将8只Wistar大鼠随机分为对照组和造模组,采用电刺激跳跃法造模,4周后处死动物,取其双后足跟腱及部分跟骨,制成观察标本,采用ImageProPlus5.0图像分析系统测定标本镜下视野照片的阳性信号积分光密度值(IOD)。结果:对照组主要在腱围处有eNOS的大量表达;造模组大鼠跟腱、纤维软骨、钙化软骨、跟骨、骨髓腔、腱骨关节面及腱围等部位eNOS表达明显,腱围及骨髓腔处表达最多。两组间不同部位IOD值均有显著差异。结果提示:eNOS可能参与了末端病组织血管的新生,组织结构功能适应及抑制细胞凋亡等代偿性反应。
OBJECTIVE: To observe the distribution of endothelial nitric oxide synthase (eNOS) in the terminal area of Achilles tendon in rats with terminal disease and to explore the relationship between eNOS and angiogenesis and its protective effect on end-stage disease. Methods: Eight Wistar rats were randomly divided into control group and model group. Animals were sacrificed after 4 weeks. The animals were sacrificed after 4 weeks and their double heel Achilles tendon and part of the calcaneus were made into observation specimens. ImageProPlus5.0 image analysis system for the determination of positron emission microscopy photos positive signal integral optical density value (IOD). Results: The rats in the control group had large numbers of eNOS expression in the tendon area. The expression of eNOS in Achilles tendon, fibrocartilage, calcified cartilage, calcaneus, bone marrow cavity, And bone marrow cavity at the most expressed. There were significant differences in IOD values between different groups in both groups. The results suggest that eNOS may be involved in the compensatory response of newborn blood vessels in end-diseased tissues, the adaptation of tissue structure and the inhibition of apoptosis.