沈抚灌区是一条以石油废水为主大型灌渠,每天接纳约40万吨污水,污水中含有大量的酚、BaP等有害物质。为了探讨污水灌田对人体健康可能产生远期的影响,我们对沈抚灌区污水进行Ames试验、微核试验等致突变性的研究。 一、材料与方法 1.污水样:对沈抚灌区污染严重的双树地区,在枯水期分别两次采水各40L。 2.污水样富集和洗脱:用Amberlite Shen-fou irrigation area is a large-scale irrigation channel dominated by irrigation water, which receives about 400,000 tons of sewage every day. The sewage contains a large amount of harmful substances such as phenol and BaP. In order to investigate the possible long-term impacts of sewage irrigation on human health, we studied the mutagenicity of sewage in the Shen-Fu irrigation area by Ames test and micronucleus test. I. Materials and Methods 1. Sewage samples: Shuangtu areas heavily polluted by Shen-Fu Irrigation District were collected 40L twice in dry season. 2. Sewage-like enrichment and elution: with Amberlite