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用烟草花叶病毒（ＴＭＶ）接种过敏性寄主枯斑三生烟或心叶烟的半叶或下部两叶，可诱导出未接种的另半叶或上部叶对ＴＭＶ再次感染的抗性，诱发接种后７ｄ用ＴＭＶ进行攻击接种，所产生的枯斑直径明显小于对照。诱发抗性最早在诱发接种后３～４ｄ被检测到，第７ｄ左右达到高峰，温度对诱发抗性的产生有很大影响。诱发接种叶上部的未接种叶因叶位不同，其抗性强弱也不同。经ＴＭＶ诱发后的心叶烟可表现出对ＴＭＶ和ＣＭＶ两者的抗性，在攻击接种ＣＭＶ后，潜育期比对照延长１～２ｄ．
Inoculation of the half leaves or lower leaves of allergic host blight trigeminal tobacco or leaf tobacco with Tobacco Mosaic Virus (TMV) induces resistance to reinfection with TMV in the unseeded second half or upper leaves Vaccination with TMV 7 days after inoculation resulted in a significantly smaller spot diameter than the control. The induced resistance was detected as early as 3 ~ 4 days after inoculation, and reached the peak around 7 days. The temperature had a great influence on the induced resistance. Inoculated leaves of the upper part of the non-inoculated leaves due to different leaf positions, the resistance is also different. After TMV-induced leaf discs tobacco can show resistance to both TMV and CMV, inoculation CMV challenge, the latent period than the control extended 1 ~ 2d.