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目的研究柴胡皂甙对原代培养肝细胞(HC)增殖及合成细胞外基质(ECM)的作用,为进一步阐明柴胡皂甙防治肝损伤和抗肝纤维化的机理提供实验依据。方法电镜观察、免疫细胞化学及图像分析仪检测HC内Ⅰ、Ⅲ、Ⅳ型胶原和纤维粘连蛋白(FN)含量,流式细胞仪测定HC内的DNA含量和3H-脯氨酸掺入量未检测HC内胶原蛋白总量。结果TKCncm各组对细胞损伤得到改善,各实验组HC内3H-脯氨酸掺入量均明显升高,各治疗组HC内DNA含量显著升高,而3H-脯氨酸掺入量均呈现降低趋势。HC内I型胶原含量明显减少,其余各组基质成分含量呈下降趋势。结论柴胡皂甙对肝细胞具有保护作用;表现为与相应实验组HC内DNA含量内呈上升趋势;胶原蛋白总量及Ⅰ、Ⅲ、Ⅳ型胶原和FN含量及其合成受到抑制,从而抑制了HC对ECM的合成
Objective To study the effects of saikosaponin on the proliferation of primary cultured hepatocytes (HC) and the synthesis of extracellular matrix (ECM), and to provide experimental evidence for further elucidating the mechanism of saikosaponin-induced liver injury and liver fibrosis. Methods The contents of type I, III, IV collagen and fibronectin (FN) in HC were detected by electron microscopy, immunocytochemistry and image analyzer. The DNA content and 3H-proline content in HC were determined by flow cytometry. The total amount of collagen in HC was measured. RESULTS: Cell damage was improved in each group of TKCncm. The incorporation of 3H-proline in HC was significantly increased in each experimental group. DNA content in HC was significantly increased in each treatment group, and 3H-proline incorporation was present. Reduce the trend. The content of type I collagen in HC decreased significantly, while the contents of matrix components in other groups decreased. Conclusion Saikosaponin has a protective effect on hepatocytes; it shows that the content of DNA in the HC of the corresponding experimental group rises; the amount of collagen and type I, III, IV collagen and FN and their synthesis are inhibited, thus inhibiting HC synthesis of ECM