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目的 研究中国大陆日本血吸虫 (Schistosoma japonicum)微卫星 DNA基因变异。方法 日本血吸虫种群取自中国大陆 7个流行省现场 :浙江省 (嘉善 ) ,安徽省 (贵池 ) ,江西省 (永修 ) ,湖北省 (武汉 ) ,湖南省 (岳阳 ) ,四川省 (茅山 ,天全 ) ,云南省 (大理 ) ,以及菲律宾 Sorsogon省。对每一种群2 0个样本的 DNA,用 6个日本血吸虫微卫星 :M5 A,J5 N,MF1,RRPS,2 AAA和 MPA分析。结果 中国大陆日本血吸虫不同的种群之间和种群内存在高水平的多态性 ,进一步证实中国株和菲律宾株分属不同虫株 ,在中国大陆不同种群间也存在明显遗传变异。结论 证明了用微卫星对日本血吸虫进行种群基因学研究的可行性 ,并提示中国大陆存在日本血吸虫不同的虫株
Objective To study the genetic variation of microsatellite DNA in Schistosoma japonicum in mainland China. Methods Schistosoma japonicum populations were collected from 7 endemic provinces in mainland China: Zhejiang (Jiashan), Anhui (Guichi), Jiangxi (Yongxiu), Hubei (Wuhan), Hunan (Yueyang), Sichuan (Maoshan, Tianquan), Yunnan (Dali), and Sorsogon Province of the Philippines. DNA from 20 samples of each population was analyzed with 6 S. japonicum microsatellites: M5 A, J5 N, MF1, RRPS, 2 AAA and MPA. Results There was a high level of polymorphism among different populations and populations of Schistosoma japonicum in mainland China. It was further confirmed that Chinese strains and Philippine strains belonged to different insect strains, and obvious genetic variation existed among different populations in mainland China. Conclusion The feasibility of population genetics studies on Schistosoma japonicum using microsatellites has been demonstrated and suggested that there are different strains of Schistosoma japonicum in mainland China