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目的运用红外光谱法和二维相关光谱技术对白芍、赤芍及其醇提物所含化学成分的红外谱图整体变化规律进行解析和鉴别。方法采用傅里叶变换红外光谱法和二维相关光谱分析技术,对白芍和赤芍及其醇提物进行鉴别分析。结果白芍、赤芍原药材一维红外光谱显现了草酸钙特征峰和位于950~1 200 cm-1处的淀粉阶梯峰,但峰形、峰位稍有差异;二阶导数处理后,二者在518/517、989 cm-1等处的特征峰得以显现,另外,赤芍在989、1 015、1 052、1 078、1 105、1 161 cm-1的峰强度均高于白芍,且峰形与白芍也不尽相同,这与两者所含淀粉的量和结构差异有关。白芍、赤芍醇提物一维光谱中都有芍药苷的特征峰(1 716、1 451、1 347、1 277、714 cm-1);图谱经二阶导数处理后可以看出两者的峰形、峰位相差较大,这与两者的糖(苷)类量和结构存在差异有关;在二维相关红外光谱中,两者在887、968、1 008、1 190、1 305 cm-1处均有糖(苷)类化合物的自动峰,不同的是白芍在1 190 cm-1处的自动峰强度最大,赤芍在968 cm-1处的自动峰强度最大,进一步佐证了两者所含糖(苷)类化合物有差异。结论红外光谱法和二维相关光谱技术提供了大量白芍、赤芍的整体结构信息,递进式地验证了两者所含物质结构和量的差异,可以初步地鉴定白芍和赤芍,为今后系统完整的鉴定工作打下基础。
OBJECTIVE: To analyze and identify the changes of the infrared spectra of the chemical constituents contained in Radix Paeoniae Alba, Radix Paeoniae Alba and its alcohol extract by infrared spectroscopy and two-dimensional correlation spectroscopy. Methods Fourier Transform Infrared Spectroscopy (FT-IR) and two-dimensional correlation spectrophotometry were used to identify the white peony root, Radix Paeoniae Rubra and its alcohol extract. Results One-dimensional IR spectra of Radix Paeoniae Alba and Radix Paeoniae Rubra showed the characteristic peak of calcium oxalate and the starch peak located at 950-1 200 cm-1, but the peak shape and peak position were slightly different. After the second derivative treatment, The peak at 518 / 517,989 cm-1 and other peaks were observed. In addition, the peak intensities of red peony root at 989,1 015,1 052,1 078,1 105,1 161 cm-1 were higher than those of white peony root , And the peak shape and white peony root are not the same, which is related to the amount and structure of starch contained in both. Paeoniae Alba, Paeonia alba extract one-dimensional spectrum of paeoniflorin characteristic peaks (1 716,1 451,1 347,1 277,714 cm-1); map by the second derivative of the treatment can be seen both The peak shape and peak position of the two peaks were different, which was related to the difference in the amount and structure of sugar (glycosides) between them. In the two-dimensional correlation infrared spectra, the two peaks were at 887,968,1 008,1 190,130 cm-1, but the difference was that the peak intensity at 1 190 cm-1 of Radix Paeoniae Alba was the highest, and that of Radix Paeoniae Rubra at 968 cm-1 was the highest The two contained sugar (glycosides) compounds are different. Conclusion Infrared spectroscopy and two-dimensional correlation spectroscopy provides a large number of white peony root, red peony’s overall structure information, and gradually verify the two substances contained in the structure and amount of differences can be initially identified white peony root and red peony root, This will lay the foundation for the complete system identification work in the future.