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一般需要5～6年才能栽培出生产黄连素的原料黄连(Coptis japonica)根,而黄连的某些悬浮培养细胞株在3周内就可生产大量的黄连素(干细胞重的8～9%)。由此启示,酶参予黄连素的生物合成。由高活性黄连细胞株64 T-33的细胞游离提取物中所产生的四氢小蘖碱是黄连素生物合成的中间体、脱氢后即形成黄连素。经HPLC 定量测定表明,231nmol 的四氢小蘖碱形成234 nmol 的黄连素,两者分子比为1:1,仅(S)-(—)-四氢小蘖碱可完全转化为黄连素,其立体异构体保持不变。由表可见,由黄细胞株(156 T-1,64 T-33)按细细胞干重计算产生250倍黄连素的酶活力比白细胞株产生低黄连素的酶活力要高100倍。此结果提示,酶在黄连素生物合成中起重要要作用,它不仅决定反应的速度,
Coptis japonica root, which produces berberine, can generally be cultivated for 5-6 years, whereas some berberine cultured cell lines produce large quantities of berberine (8-9% of stem cell weight) within 3 weeks. . Inspired by this, enzymes involved in berberine biosynthesis. Produced by cell free extract of high activity Coptis cell line 64 T-33, the produced tetrahydrobiopterin is an intermediate of the biosynthesis of berberine. After dehydrogenation, berberine is formed. Quantitative determination by HPLC showed that 231 nmol of tetrahydrobiopterin formed 234 nmol of berberine at a molecular ratio of 1: 1, and only (S) - (-) - tetrahydrobiopterin could be completely converted to berberine, Its stereoisomers remain unchanged. It can be seen from the table that the activity of producing 250-fold berberine from the yellow cell strain (156 T-1,64 T-33) is 100 times higher than that of the low-berberine producing strain of white blood cell strain. This result suggests that the enzyme plays an important role in the biosynthesis of berberine, which not only determines the speed of the reaction,