论文部分内容阅读
用中性红吸收分析法和放射配体结合法分析基因重组干扰素－α（ｒＩＦＮ－α）对胃癌细胞（ＳＧＣ７９０１、ＫＡＴＯⅢ）增殖及其表皮生长因子受体（ＥＧＦ－Ｒ）表达的影响。结果显示ｒＩＦＮ－α可明显抑制ＳＧＣ７９０１细胞增殖，与作用时间、剂量相关，而对ＫＡＴＯⅢ增殖无明显抑制作用。采用氯胺Ｔ法自行标记表皮生长因子（ＥＧＦ），配体结合实验Ｓｃａｔｃｈａｒｄ分析结果ＳＧＣ７９０１、ＫＡＴＯⅢ均高表达单一亲和性的ＥＧＦ－Ｒ。ｒＩＦＮ－α降低ＳＧＣ７９０１细胞ＥＧＦ－Ｒ数量，与作用时间、剂量相关，ＥＧＦ－Ｒ亲和性无变化，而对ＫＡＴＯⅢ细胞ＥＧＦ－Ｒ的数量及亲和性均无明显影响。结果提示ｒＩＦＮ－α抑制胃癌细胞生长可能和其下调细胞ＥＧＦ－Ｒ相关。
The effects of recombinant interferon-α(rIFN-α) on the proliferation of gastric cancer cells (SGC7901, KATOIII) and the expression of epidermal growth factor receptor (EGF-R) were analyzed by neutral red absorption assay and radioligand binding assay. The results showed that rIFN-α can significantly inhibit the proliferation of SGC7901 cells, which is related to the action time and dose, but has no obvious inhibitory effect on the proliferation of KATOIII. Self-labeling of epidermal growth factor (EGF) was performed using the chloramine T method. Scatchard analysis of ligand binding assays showed that SGC7901 and KATOIII all expressed high affinity EGF-R. rIFN-α decreased the number of EGF-R in SGC7901 cells, and was related to the time and dose. The affinity of EGF-R did not change, but there was no significant effect on the number and affinity of EGF-R in KATO III cells. The results suggest that rIFN-α inhibits the growth of gastric cancer cells may be related to its downregulation of EGF-R cells.