论文部分内容阅读
采用基于16 SrDNA的变性梯度凝胶电泳(DGGE)的方法,研究了转Btcry1Ac基因棉花SGK321对益虫——意大利蜜蜂(Apis mellifera ligustica)肠道细菌群落的影响.分别用转基因/非转基因棉花花粉和高浓度外源DNA饲喂蜜蜂7d,通过DGGE分析经不同处理的蜜蜂肠道细菌群落组成的差异,同时用PCR检测是否发生外源基因的横向转移.结果显示,各个处理组DGGE图谱中的条带数目在11.3～15.0之间,Shannon’s指数在0.87～1.05之间,相似性系数介于68.3%～84.7%,不同处理组肠道细菌组成差异不明显,用PCR的方法也没有检测到外源基因的横向转移.因此初步判定转基因棉花SGK321短期内对蜜蜂细菌群落尚无不利影响.图2表3参22
The effect of transgenic Btcry1Ac cotton SGK321 on the intestinal bacterial community of the beneficial insect Apis mellifera ligustica was studied by using 16 SrDNA denaturing gradient gel electrophoresis (DGGE). The transgenic cotton plants were treated with transgenic / non-transgenic cotton pollen and High concentrations of exogenous DNA were applied to bees for 7 days, and DGGE was used to analyze the differences in intestinal bacterial community composition of the bees treated by different treatments. PCR was also performed to detect the lateral metastasis of exogenous genes. The results showed that the DGGE profiles of each treatment group The numbers of bands ranged from 11.3 to 15.0, Shannon’s index ranged from 0.87 to 1.05, and the similarity coefficients ranged from 68.3% to 84.7%. There were no significant differences in the bacterial composition of the gut in different treatment groups, and no exogenous The horizontal transfer of the gene.Therefore, the preliminary determination of transgenic cotton SGK321 short-term within the honeybee bacterial community has no adverse effect.Figure 2 Table 3 参 22