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一、样品化学发光的估算 与淬灭现象相反,样品化学发光在液闪测量中会导致假计数。为此,在每次建立新的样品制备方法之后都需估计该方法是否会导致化学发光,若有,还要估计它对测量结果影响的程度。已有多种检测化学发光的方法报道,但大多有各自的缺点:用不含放射性的对照样品的方法则比较费时间:用双重外标准源道比法需专门绘制一条标准曲线且在淬灭严重时检测有困难;用延迟符合的技术则需要增加电子学线路。在我们的配接了MIC-80微机的YSJ型液闪仪上,当进行化学发光检测时,仪器会自动给出样品化学发光的估算值nc。若nc=0,则可肯定样品无化学发光或者说化学发光对测量结果无影响;若nc≠0,则提示我们,该样品可能有化学发光存
First, the estimated chemiluminescence of the sample Contrary to the phenomenon of quenching, the chemiluminescence of the sample results in false counts in the measurement of the liquid flashes. For this reason, it is necessary to estimate whether this method will cause chemiluminescence after each new sample preparation method is established, and if so, to estimate the extent to which it affects the measurement results. A variety of chemiluminescent chemiluminescence detection methods have been reported, but many have their own drawbacks. The method of using radioactive control samples takes a long time: a standard curve is drawn exclusively by the double outer standard source ratio method, Detection of serious difficulties; delay with the technology you need to increase the electronics line. On our YSJ flasher with a MIC-80 computer, the instrument automatically gives an estimate of the chemiluminescence of the sample, nc, when performing chemiluminescence detection. If nc = 0, you can be sure that the sample without chemiluminescence or chemiluminescence on the measurement results have no effect; if nc ≠ 0, then prompt us that the sample may have chemiluminescence