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Mueller(1975)提出,淋巴细胞酸性酯酶染色法(Acid a—naphthylacetate esterase(ANAE),可以作为小鼠静止成熟的T细胞标志之一;以后Knowles等(1978)又将该实验方法应用于人类T淋巴细胞的检查。以后ANAE很快被采纳推广。目前国内外已有许多这方面的报导。但由于ANAE受影响的因素较多,往往得不到令人满意的染色效果或染色效果不稳定,因之在推广应用方面受到一定限制。我们在研究小鼠淋巴细胞ANAE过程中,对传统的ANAE法作了适当修改,取得了较好的效果,现将方法介绍如下:
Mueller (1975) proposed that Acid a-naphthylacetate esterase (ANAE) can be used as one of the markers of immature and mature T cells in mice; later, Knowles et al. (1978) applied the experimental method to humans. After the examination of T-lymphocytes, ANAE was quickly adopted and promoted.There have been many reports in this area at home and abroad, but because of the many factors that affect the AAE, they often cannot obtain satisfactory dyeing effects or unstable dyeing effects. Therefore, due to its limited application in promotion and application, we have made appropriate modifications to the traditional ANAE method during the study of the mouse ANAE lymphocyte process and achieved good results. The method is now described as follows: