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目的 观察恒定磁场诱导下阿霉素磁性蛋白微球体外对恶性肿瘤细胞生长的抑制作用。方法 体外培养的Walker 2 5 6细胞分为阿霉素磁性微球联合磁场组 ,阿霉素磁性微球组及阿霉素组三组 ,倒置显微镜观察细胞的生长状态 ,改良MTT法检测各组细胞的抑制率及半数抑制剂量 ,3H TdR掺入法检测各组抑制率。结果 阿霉素磁性微球组与游离阿霉素组抑制率及半数抑制剂量相似 (P >0 .0 5 ) ,联合磁场组抑制率明显提高 ,半数抑制剂量约降低一半 (P <0 .0 1) ,两种检测方法的药敏试验相符率达 84.19% ,细胞形态出现显著变化。结论 阿霉素磁性蛋白微球与游离阿霉素对恶性肿瘤细胞具有相似的抑制作用 ,联合恒定磁场可增强其抑瘤效应。
Objective To observe the inhibitory effect of adriamycin magnetic protein microspheres on malignant cell growth in vitro induced by a constant magnetic field. Methods Walker 256 cells cultured in vitro were divided into adriamycin magnetic microspheres combined magnetic field group, doxorubicin magnetic microsphere group and doxorubicin group. The growth status of cells was observed by inverted microscope, and the improved MTT assay was used to detect each group. The inhibitory rate of the cells and the amount of half inhibitors were measured by 3H TdR incorporation method. Results The inhibition rate and half inhibitory dose of doxorubicin magnetic microspheres group and free doxorubicin group were similar (P > 0.05). The inhibition rate of combined magnetic field group was significantly increased, and the half inhibitory dose was reduced by about half (P <0. 1) The coincidence rate of susceptibility testing with the two detection methods reached 84.19%, and the cell morphology changed significantly. Conclusion Adriamycin magnetic protein microspheres and free doxorubicin have similar inhibitory effects on malignant tumor cells, and combined with a constant magnetic field can enhance its antitumor effect.