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前期研究发现分离自毛竹的小孢拟盘多毛孢Pestalotiopsis m icrosporaPM-1菌株具有较强的除草活性,为了明确其产生除草活性物质的最佳液体发酵条件,对培养基种类及成分、培养温度、培养时间和培养方式进行了探讨,并利用薄层层析(thin-layer chromatography,TLC)和高效液相色谱(high performance liquid chromatography,HPLC)对除草活性物质进行了分离。小孢拟盘多毛孢在含硫酸亚铁的PD培养基中,30℃黑暗静止培养15天,得到的粗提物除草活性最强;除草活性物质经TLC分离获得7个条带,其中条带6具有较强的除草活性,HPLC分析发现该条带含有7个组分,其中组分3对马唐的活性较强,其保留时间为46.3 m in。研究结果表明,利用最佳液体发酵条件对PM-1菌株进行发酵培养,可分离获得除草活性化合物——组分3。
The preliminary study found that Pestalotiopsis m icrosporaPM-1 strain isolated from Phyllostachys pubescens had strong herbicidal activity. In order to clarify the optimal liquid fermentation conditions for its production of herbicidal active substances, the species and composition of culture medium, culture temperature, Culture time and culture method were studied. The herbicidal active substances were separated by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). Microsporum in the PD medium containing ferrous sulfate, cultured in darkness at 30 ℃ for 15 days, the herbicidal activity of the obtained crude extract was strongest. Seven bands of the herbicidal active substance were obtained by TLC separation. The bands 6 has strong herbicidal activity. HPLC analysis showed that the band contains seven components, of which component 3 has a strong activity on crabgrass with a retention time of 46.3 mins. The results showed that the herbicidal active compound - component 3 could be isolated and obtained by fermenting PM-1 strain with the best liquid fermentation conditions.