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目的研究双氢青蒿素(DHA)对体外培养的人肝癌细胞株HepG2的生长抑制作用及诱导凋亡作用。方法常规体外培养人肝癌细胞株HepG2,将不同浓度DHA在不同时间内作用于人肝癌细胞株HepG2,采用四甲基偶氮唑盐比色法(MTT)检测细胞生长抑制率,应用流式细胞仪检测细胞凋亡情况。结果不同浓度DHA均可抑制体外培养的人肝癌细胞株HepG2细胞生长,并可诱导其发生凋亡,且呈一定的剂量-效应、时间-效应关系。结论 DHA对体外培养的人肝癌细胞株HepG2有明显的生长抑制作用及诱导凋亡作用。
Objective To study the inhibitory effect of dihydroartemisinin (DHA) on the growth of human hepatocellular carcinoma cell line HepG2 and the induction of apoptosis. Methods Human hepatocellular carcinoma cell line HepG2 was cultured in vitro. Different concentrations of DHA were applied to HepG2 cells in different time periods. Cell growth inhibition rate was determined by MTT assay. Flow cytometry Instrument detection of apoptosis. Results DHA at different concentrations could inhibit the growth of HepG2 cells in vitro and induce apoptosis of HepG2 cells in vitro. The results showed dose-effect and time-effect relationship. Conclusion DHA has obvious growth inhibitory effect and apoptosis inducing effect on HepG2 cell line cultured in vitro.