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构建重组腺病毒载体 Ad CMVp53 ,将 wtp53分别导入 PG(人肺巨细胞瘤细胞 )、CAE(人结肠癌细胞 )、HCT(人结肠癌细胞 )、He La(人宫颈癌细胞 )及 BEL74 0 2 (人肝癌细胞 )五种癌细胞中 ,测定 Ad CMVp53对癌细胞的半抑制浓度 ( IC5 0 )和细胞生长曲线 ,分析不同组织癌细胞对 Ad CMVp53的敏感程度 .结果表明 ,Ad CMVp53对癌细胞具有与病毒剂量相关的明显致死作用 ,但不同细胞对腺病毒剂量的敏感程度有差异 ,PG、CAE和BEL74 0 2三个细胞系较为敏感 ,其 IC5 0 低于 1 5MOI(重复感染率 ,multipicity of infection) ;而 He La和 HCT细胞需要较高的病毒剂量 ,IC5 0 分别为 1 0 0 MOI和 80 0 MOI.
Recombinant adenovirus Ad CMVp53 was constructed and wtp53 was introduced into PG (human lung giant cell tumor cell), CAE (human colon cancer cell), HCT (human colon cancer cell), He La (human cervical cancer cell) and BEL74002 (IC50) and cell growth curve of AdCMVp53 were detected in five kinds of cancer cells (human hepatoma cells), and the sensitivity of AdCMVp53 cells in different tissues was analyzed.The results showed that Ad CMVp53 could effectively inhibit the proliferation of cancer cells With significant lethal effect related to the dose of virus, but different cells have different sensitivity to adenovirus dose. The three cell lines of PG, CAE and BEL74O2 are more sensitive with IC50 lower than 15MOI (multiplicity of infection of infection); whereas He La and HCT cells require higher viral doses with IC50 values of 100 MOI and 800 MOI, respectively.