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背景:胆碱能神经系统存在胆碱与神经生长因子受体酪氨酸激酶A共同作用的位点,胆碱能损伤是否影响到酪氨酸激酶A的变化,对于能否应用神经生长因子干预认知障碍疾病具有重要意义。目的押观察双侧穹窿海马伞切断大鼠脑内不同部位酪氨酸激酶A表达的变化,了解穹窿海马伞切断对神经生长因子受体系统的影响。设计:以动物为观察对象,完全随机对照实验。单位:青岛大学医学院附属医院脑血管病研究所。材料:实验于2003-03/12在青岛大学医学院附属医院脑血管病研究所完成。14只雌性Wistar大鼠随机分为对照组、模型组两组,每组7只。方法押模型组大鼠行双侧穹窿海马伞切断,对照组同样手术,但不切断穹窿海马伞。两组大鼠术后观察28d后麻醉状态下处死,取脑,行免疫组化染色。主要观察指标:两组大鼠脑海马CA1区、皮质区、杏仁复合体区、基底前脑Meynert核区酪氨酸激酶A阳性细胞数量。结果押14只大鼠全部进入结果分析。模型组大鼠大脑皮质区、脑海马CA1区、杏仁复合体区、Meynert核区酪氨酸激酶A阳性细胞数显著少于对照组眼穴18.91±6.27雪,(15.17±5.23),(18.71±9.05),(8.03±2.33)个;(54.77±11.84),(59.69±10.40),(49.23±15.84),(21.49±15.54)个,t=4.17～10.00鸦P<0.01演。结论押穹窿海马伞切断大鼠脑内多部位酪氨酸激酶A表达减少,这种病理变化可能是认知障碍和老年性痴呆的原因之一。
BACKGROUND: The cholinergic nervous system has a site of interaction of choline with the nerve growth factor receptor tyrosine kinase A, and whether cholinergic injury affects tyrosine kinase A changes. Whether or not nerve cell growth factor intervention can be applied Cognitive disorders are of great importance. Objective To observe the change of tyrosine kinase A expression in different parts of the brain of rats with bilateral fimbria fornix transection to understand the influence of fimbria fornix transection on the nerve growth factor receptor system. Design: The animals as the observation object, a completely randomized controlled experiment. Unit: Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University Medical College. Materials: The experiment was performed at the Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University Medical College from March to December 2003. Fourteen female Wistar rats were randomly divided into control group, model group, two groups, seven in each group. Methods The rats in model group were subjected to bilateral fimbria fornix transection, while those in control group were operated under the same condition, but did not cut the fimbria fornix. The rats in both groups were sacrificed 28 days after anesthesia, and the brain and immunohistochemistry were performed. MAIN OUTCOME MEASURES: The number of tyrosine kinase A positive cells in hippocampal CA1 region, cortex, almond complex and Meynert nucleus in the basal forebrain in both groups. Results All 14 rats were involved in the result analysis. The number of tyrosine kinase A positive cells in cerebral cortex, hippocampus CA1, almond complex and Meynert nucleus in model group was significantly lower than that in control group (18.91 ± 6.27, (15.17 ± 5.23), (18.71 ± 9.05), (8.03 ± 2.33), (54.77 ± 11.84), (59.69 ± 10.40), (49.23 ± 15.84), (21.49 ± 15.54), t = 4.17-10.00. Conclusions The expression of tyrosine kinase A in multiple parts of the brain in rats after fimbria fornix transection can be reduced. This pathological change may be one of the reasons of cognitive impairment and senile dementia.