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本室在乳鼠心肌细胞培养的基础上,最近又成功地培养了人胚心肌细胞。现报告如下。材料和方法妊娠17及18周的胎儿,经水囊引产娩出后,立即在无菌条件下取出心室肌,剪碎,以0.06％胰蛋白酶分次消化成单细胞,用含20％小牛血清的Eagle培养基(MEM)制成10~6细胞/毫升的心肌细胞悬液,进行原代人胚心肌细胞单层培养。在倒置显微镜下观察心肌细胞的搏动及形态,拍摄照片,以及录制心肌细胞搏动的电视录像,并作扫描也子显微镜的观察。
Based on the culture of neonatal cardiomyocytes, we successfully cultured human embryonic cardiomyocytes recently. The report is as follows. MATERIALS AND METHODS Fetuses at 17 and 18 weeks of gestation were taken out of the abdominis of the water sac immediately after aseptic delivery. The ventricular muscles were removed aseptically, cut into pieces, digested with 0.06% trypsin into single cells, and stained with 20% fetal bovine serum Of Eagle’s medium (MEM) to make a suspension of 10-6 cells / ml of cardiomyocytes for monolayer culture of primary human embryonic cardiomyocytes. Under inverted microscope, the beating and morphology of cardiomyocytes were observed, photographs were taken, and video recordings of cardiomyocytes pulsation were recorded. Scanning and sub-microscopic observations were also performed.