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目的纵向研究高效抗逆转录病毒治疗(HAART)对HIV单一感染者和HIV/HCV合并感染者外周血单个核细胞(PBMCs)中粘病毒抵抗蛋白Mx A mRNA表达水平的影响,分析HAART后HIV/HCV合并感染者抗HCV前基线Mx A mRNA水平是否与后续干扰素抗HCV疗效有关。方法以广州市第八人民医院收治的艾滋病患者(HIV单一感染组,HAART前CD4+T淋巴细胞<200个/mm3,42例)和HIV/HCV合并感染患者(HIV/HCV合并感染组,HAART前CD4+T淋巴细胞<200个/mm3,33例)为研究对象,并以22名健康自愿者作为对照;观察患者在抗HCV治疗前的HAART的不同时间点(0 W、4 W、12 W、24 W、48 W、72 W、96 W)PBMCs中Mx A mRNA表达水平;对比分析后继抗HCV治疗效果(有早期病毒学应答EVR,无早期病毒学应答NEVR)与Mx A mRNA表达水平的关系。结果 HIV单一感染组PBMCs中Mx A mRNA水平在HAART 4 W后下降(P<0.05),其他各时间点及与健康对照组之间差异均无统计学意义(P>0.05);HIV/HCV合并感染组PBMCs中HAART各时间点及与健康对照组之间差异均无统计学意义(P>0.05);HAART 96 W后进行抗HCV治疗,EVR组抗HCV治疗前Mx A mRNA水平较NEVR组高(P<0.05)。结论长期HAART对HIV/HCV合并感染者PBMCs中Mx A mRNA表达水平无显著影响;但HAART后抗HCV治疗前PBMCs中Mx A mRNA水平可作为HIV/HCV合并感染者抗HCV疗效的预测指标之一。
Objective To investigate the effect of HAART on the expression of myxomagglutinin Mx A mRNA in peripheral blood mononuclear cells (PBMCs) infected with HIV and HCV / HCV co-infected with HIV, Whether anti-HCV pre-HCV Mx A mRNA levels in HCV co-infected patients is associated with the efficacy of subsequent interferon anti-HCV. Methods HIV positive patients (HIV infected group, HAART positive CD4 + T lymphocytes <200 / mm3, 42 cases) and HIV / HCV co-infected patients Pre-CD4 + T lymphocytes (<200 / mm3, 33 cases) were selected as the control subjects and 22 healthy volunteers served as controls. Patients were observed at different time points (0 W, 4 W, 12 W, 24 W, 48 W, 72 W, 96 W). The levels of Mx A mRNA expression in PBMCs were analyzed by comparative analysis of the anti-HCV treatment effect (early virological response to EVR and no early virological response to NEVR) Relationship. Results The levels of Mx A mRNA in PBMCs infected with HIV single infection decreased after 4 weeks of HAART (P <0.05), and there was no significant difference at other time points and healthy controls (P> 0.05). HIV / HCV combined There was no significant difference between HAART group and control group (P> 0.05). Anti-HCV treatment was performed after 96-h HAART, and the level of Mx A mRNA in EVR group was higher than that in NEVR group (P <0.05). Conclusion Long-term HAART has no significant effect on the expression of Mx A mRNA in PBMCs infected with HIV / HCV co-infection. However, the level of Mx A mRNA in PBMCs pre-HAART anti-HCV treatment may be one of the predictors of anti-HCV efficacy in HIV / HCV co-infected patients .