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目的探明3种因素对金线莲Anoectochilus roxburghii丛生芽生物量和有效物质积累的影响以及在反应器内通过大量培养丛生芽生产有效物质的可行性,为金线莲产品开发提供一种新的材料来源。方法以金线莲丛生芽为材料,研究外植体长度、BA质量浓度和光条件对金线莲丛生芽的影响,观察利用气升式生物反应器培养金线莲丛生芽对生物量和有效物质积累的影响。结果外植体大小为5 mm时丛生芽增殖效果显著好于1 mm。BA质量浓度为2.5 mg/L时生物量最高,但丛生芽中多糖、黄酮和总酚等有效物质的生产量在BA质量浓度为2.0 mg/L时出现最高值。明培养比暗培养更适于生物量和有效物质的积累。反应器培养的丛生芽生物量,多糖,黄酮和酚类物质的生产量(678.3、12.2、17.8 mg/L)明显高于固体培养。结论外植体长度为5 mm、BA质量浓度为2.0 mg/L、在明培养条件下,利于丛生芽的生长,可生产较多的多糖、黄酮和酚类物质。在短时间内通过气升式生物反应器可得到大量丛生芽,反应器培养比固体培养能获得更多的生物量和有效物质。
Objective To explore the effects of three factors on biomass and bioaccumulation of clustered buds of Anoectochilus roxburghii and the feasibility of producing effective substances through massive culture of cluster buds in Anoectochilus roxburghii so as to provide a new Material source. Methods The buds of A. gossypii were used to study the effects of explant length, BA concentration and light conditions on the bud formation of A. amurensis. The effects of biomass, active substance The impact of accumulation. Results The proliferation of clustered buds was significantly better than 1 mm when explant size was 5 mm. The biomass of BA was the highest at 2.5 mg / L BA, but the production of effective substances such as polysaccharides, flavonoids and total phenols in culm appeared highest when the concentration of BA was 2.0 mg / L. Ming training is more suitable than the dark culture biomass and the accumulation of effective substances. The biomass of the shoots, the production of polysaccharides, flavonoids and phenols (678.3, 12.2, 17.8 mg / L) were significantly higher than that of the solid culture. Conclusion The length of explant is 5 mm and the concentration of BA is 2.0 mg / L. Under the culture condition, it is good for the growth of clumpy shoots and more polysaccharides, flavonoids and phenols can be produced. In a short period of time through the airlift bioreactor can be a large number of clusters of buds, reactor culture than solid culture can get more biomass and effective substances.