论文部分内容阅读
目的:探讨Nogo-A在痛觉调制过程中的作用以及它在大鼠中脑导水管周围灰质(periaqueductal gray,PAG)内与降钙素基因相关肽(CGRP)和内源性阿片系统在痛觉调制过程中的相互关系。方法:选用雄性SD大鼠32只,随机等分为4组:其中空白对照组8只,分别向PAG内注射0.9%生理盐水1μl;痛敏模型对照组、吗啡组和纳洛酮组各8只,首先单侧后爪掌心皮下注射1%的福尔马林0.1 ml复制痛敏大鼠模型,3 d后分别向PAG内注射等量0.9%生理盐水、吗啡和纳洛酮各1μl。60 min后取大鼠大脑PAG区,采用免疫组化法观测各组大鼠PAG内CGRP和Nogo-A免疫反应阳性神经元的表达差异。结果:与空白对照组比较,模型对照组大鼠PAG内CGRP的表达量显著增高(P<0.01),Nogo-A显著降低(P<0.01);与模型对照组比较,吗啡组大鼠PAG内CGRP的表达量显著降低(P<0.05),Nogo-A显著增高(P<0.01),而纳洛酮组PAG中CGRP的表达量显著增高(P<0.01),Nogo-A显著降低(P<0.05)。结论:Nogo-A和CGRP参与了痛觉调制过程,但Nogo-A与CGRP之间有反向调节作用;Nogo-A与内源性阿片系统之间可能也存在一定的功能联系。
AIM: To investigate the role of Nogo-A in pain modulation and its association with calcitonin gene-related peptide (CGRP) and endogenous opioid system in the regulation of pain modulation in rat midbrain periaqueductal gray (PAG) The process of interrelationship. Methods: Thirty-two male Sprague-Dawley rats were randomly divided into 4 groups: control group, n = 8, blank control group injected 0.9% saline into PAG respectively, and control group, morphine group and naloxone group Only the unilateral paw palmar was subcutaneously injected with 0.1% formalin (1 ml) into a painful rat model. After 3 days, 0.9% saline, morphine and naloxone were injected into the PAG respectively. The PAG region of the rat brain was taken after 60 min. The expression of CGRP and Nogo-A immunoreactive neurons in the PAG of each group were observed by immunohistochemistry. Results: Compared with the blank control group, the expression of CGRP in PAG was significantly increased (P <0.01) and Nogo-A was significantly decreased in model control group (P <0.01). Compared with model control group, The expression of CGRP was significantly lower (P <0.05) and Nogo-A was significantly higher (P <0.01), while the expression of CGRP in NAG group was significantly higher (P <0.01) and Nogo-A was significantly lower 0.05). CONCLUSION: Nogo-A and CGRP are involved in the process of pain modulation. However, Nogo-A and CGRP play an inverse regulatory role. Nogo-A and endogenous opioid system may also have some functional relationship.