论文部分内容阅读
目的通过体外实验研究伊犁黑蜂蜂胶对牙釉质脱矿和再矿化的作用,评价其作用效果。方法应用离体牛切牙制备釉质样本60个,随机分为3组:氟化钠组(阳性对照)、去离子水组(阴性对照)和蜂胶组。分别进行体外脱矿和再矿化实验,应用扫描电镜观察釉质表面形态,采用激光共聚焦扫描显微镜观察釉质脱矿程度,通过测量釉质切片的荧光面积、总荧光量和病损平均荧光量对抑制脱矿和促进再矿化的效果进行量化,采用单因素方差分析进行统计学分析。结果扫描电镜观察示:脱矿后三组都可见釉质表面不平整,但氟化钠组和蜂胶组可见釉质表面颗粒的再附着,去离子水组釉质表面凹凸不平且未见颗粒附着;再矿化后三组釉质表面都有不同程度的形态恢复,氟化钠组的釉质表面较脱矿后更加平整,有许多圆形或椭圆形的细密颗粒沉积,而去离子水组和蜂胶组仍可见釉柱中心发生脱矿所形成的凹坑。激光共聚焦扫描显微镜观察示:脱矿后氟化钠组和蜂胶组的荧光面积、总荧光量和平均荧光量较去离子水组均小,差异有统计学意义(P<0.05);蜂胶组的荧光面积、总荧光量和平均荧光量均较氟化钠组大,作用虽弱于氟化钠组,但差异不具有统计学意义(P>0.05)。再矿化后氟化钠组的荧光面积、总荧光量和平均荧光量较去离子水组和蜂胶组均小,差异有统计学意义(P<0.05);蜂胶组的荧光面积、总荧光量和平均荧光量虽均较去离子水组小,但差异不具有统计学意义(P>0.05)。结论伊犁黑蜂蜂胶能够有效抑制牙釉质脱矿,但不具备促进脱矿牙釉质再矿化的作用。
Objective To study the effect of Ili black bee propolis on demineralization and remineralization of enamel in vitro and to evaluate its effect. Methods Sixty enamel samples were obtained from bovine incisors and were randomly divided into three groups: sodium fluoride group (positive control), deionized water group (negative control) and propolis group. In vitro demineralization and remineralization experiments were performed. The surface morphology of enamel was observed by scanning electron microscopy. The degree of enamel demineralization was observed by laser scanning confocal microscopy. The fluorescence area, total fluorescence and average fluorescence of enamel slices were measured. Demineralization and the promotion of remineralization were quantified using one-way analysis of variance for statistical analysis. Results Scanning electron microscopy showed that the enamel surface was irregular after demineralization. However, the attachment of enamel surface particles was observed in sodium fluoride group and propolis group. The surface of enamel in deionized water group was uneven and no particles were attached. After enamel treatment, the enamel surface restored to some extent. The enamel surface of sodium fluoride group was smoother than that after demineralization. Many round or oval fine particles were deposited, while the deionized water group and propolis group were still visible Glaze column center occurred demineralization formed by the pit. Laser scanning confocal microscopy showed that the fluorescence, total fluorescence and average fluorescence of the demineralized sodium fluoride and propolis groups were lower than those of the deionized water group (P <0.05) The fluorescence area, the total amount of fluorescence and the average amount of fluorescence were larger than the sodium fluoride group, although the effect was weaker than the sodium fluoride group, but the difference was not statistically significant (P> 0.05). After remineralization, the fluorescence area, total fluorescence and average fluorescence of sodium fluoride group were smaller than that of deionized water group and propolis group (P <0.05), and the fluorescence area and total fluorescence of propolis group Although the average fluorescence intensity was lower than that of deionized water group, the difference was not statistically significant (P> 0.05). Conclusion Ili black bee propolis can effectively inhibit the demineralization of enamel, but does not have the effect of promoting remineralization of demineralized enamel.