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目的:探讨利心冲剂对心衰大鼠模型心肌细胞能量代谢的影响。方法:腹腔注射阿霉素(ADR)复制大鼠心衰模型,观察心肌细胞形态学变化,Real-timePCR定量检测过氧化物酶体增生物激活受体协同刺激因子-1(PGC-1α)的表达,免疫组化检测雌激素受体α(ERα)的表达。结果:利心冲剂治疗组心肌细胞变性坏死明显减轻,PGC-1α、ERα的表达明显增加。结论:利心冲剂可改善大鼠心衰模型心肌细胞的能量代谢异常,并改善心功能。
Objective: To investigate the effect of Lixin granules on the energy metabolism of cardiomyocytes in heart failure rat model. Methods: Adriamycin (ADR) was injected intraperitoneally to establish a rat model of heart failure. The morphological changes of myocardial cells were observed. Real-time PCR was used to detect the expression of peroxisome proliferator-activated receptor co-stimulatory factor-1 (PGC-1α) The expression of estrogen receptor alpha (ERα) was detected by immunohistochemistry. Results: The cardiomyocyte degeneration and necrosis in the Lixin granules group were significantly reduced, and the expressions of PGC-1α and ERα were significantly increased. Conclusion: Lixin granule can improve the energy metabolism of cardiomyocytes in heart failure model rats and improve cardiac function.