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目的建立水提醇沉法提取蒲葵子多糖的最佳提取工艺。方法采用正交试验,以多糖含量为指标,优选提取时间(A)、提取次数(B)、粒度(C)。结果影响蒲葵子多糖含量的因素主次关系C>A>B,最佳提取工艺为A2B3C2。结论药材粒度对结果有显著影响,从经济、省时的角度考虑,生产上应把药材的粒度作为关键性因素考虑。
OBJECTIVE To establish the optimum extraction process of polysaccharides extracted by water extraction and alcohol precipitation. Methods The orthogonal test was used to determine the polysaccharides content. The optimum extraction time (A), extraction times (B) and particle size (C) were obtained. Results Primary and secondary factors that affected the content of polysaccharides in P. ciliata were C> A> B and the best extraction was A2B3C2. Conclusion The particle size of medicinal materials has a significant effect on the results. Considering the economic and time-saving aspects, the particle size of medicinal materials should be considered as the key factor in the production.